Title: Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis


Authors: Qing Cao1, Yan Shen1, Wei Zheng1, Hao Liu1, Chen Liu2


Institutions: 1College of Medicine, Henan University of Science and Technology, Luoyang, Henan 471023, China; 2Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 211166, China.


Abstract: Kruppel-like factor 4 (Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis. However, its regulation during embryogenesis is still unclear. Here, we report that Tcf7l1, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf7l1. Moreover, the dominant negative form of Tcf7l1 (dnTcf7l1), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent. Taken together, our results demonstrate that Tcf7l1 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.


Keywords: Kruppel-like factor 4 (Klf4), Tcf7l1, transcription regulation, Xenopus laevis


Full Text: JBR-2017-0056.pdfJBR-2017-0056-supplementary.pdf


J Biomed Res published on 30 November 2017, https://doi.org/10.7555/JBR.32.20170056